Microplitis bicoloratus Bracovirus Promotes Cyclophilin D-Acetylation at Lysine 125 That Correlates with Apoptosis during Insect Immunosuppression

Cyclophilin D (CypD) is regulated during the innate immune response of insects. However, mechanism by which CypD activated under immunosuppression not understood. Microplitis bicoloratus bracovirus (MbBV), a symbiotic virus in parasitoid wasp, bicoloratus, suppresses immunity parasitized Spodoptera litura. Here, we demonstrate that MbBV promotes acetylation S. litura, resulting an immunosuppressive phenotype characterized increased apoptosis hemocytes and MbBV-infected cells. Under infection, inhibition significantly rescued apoptotic cells induced MbBV, point-mutant fusion proteins CypDK125R-V5 were deacetylated. The CypD-V5 acetylated Deacetylation can also suppress MbBV-induced increase apoptosis. These results indicate involved MbBV-suppressed via CypD-acetylation pathway litura on K125.